Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria

• Published in: Biosensors & bioelectronics Vol. 30; no. 1; pp. 67 - 72
• Main Authors: Zhou, Jin, Tun, Thet Naing, Hong, Sung-ha, Mercer-Chalmers, June D., Laabei, Maisem, Young, Amber E.R., Jenkins, A. Tobias A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.12.2011; Elsevier
• Subjects: Bacterial Load - instrumentation; Bacterial Load - methods; Bandages; Biological and medical sciences; Biosensing Techniques - instrumentation; Biotechnology; Colorimetry - instrumentation; Equipment Design; Equipment Failure Analysis; Escherichia coli; Fundamental and applied biological sciences. Psychology; Humans; Infection; Phospholipid vesicle; Pilot Projects; Pseudomonas aeruginosa; Staphylococcus aureus; Wound Infection - diagnosis; Wound Infection - microbiology; Wound Infection - therapy; Wound-dressing; Infection; Wound-dressing; Pseudomonas aeruginosa; Phospholipid vesicle; Staphylococcus aureus; Pseudomonadales; Vesicle; Phospholipid; Technology; Pathogenic; Micrococcales; Pseudomonadaceae; Bacteria; Micrococcaceae; Colonization
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2011.08.028

A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin) and lipases (phospholipase A 2). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5α in a prototype dressing.