AhNRAMP1 iron transporter is involved in iron acquisition in peanut

• Published in: Journal of experimental botany Vol. 63; no. 12; pp. 4437 - 4446
• Main Authors: Xiong, Hongchun, Kobayashi, Takanori, Kakei, Yusuke, Senoura, Takeshi, Nakazono, Mikio, Takahashi, Hirokazu, Nakanishi, Hiromi, Shen, Hongyun, Duan, Penggen, Guo, Xiaotong, Nishizawa, Naoko K, Zuo, Yuanmei
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press [etc.] 01.07.2012; Oxford University Press
• Subjects: agroecosystems; Agronomy. Soil science and plant productions; Arachis - genetics; Arachis - metabolism; Arachis - physiology; Base Sequence; Biological and medical sciences; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; chlorosis; continuous cropping; corn; Cropping systems. Cultivation. Soil tillage; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Plant - physiology; General agronomy. Plant production; Generalities. Cropping systems and patterns; genes; Genetic Complementation Test; in situ hybridization; intercropping; iron; Iron - metabolism; Iron Deficiencies; leaves; Membrane Proteins - genetics; Membrane Proteins - metabolism; messenger RNA; Molecular Sequence Data; Mutation; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - physiology; nutrient deficiencies; peanuts; Phylogeny; Plant Epidermis - embryology; Plant Epidermis - genetics; Plant Epidermis - physiology; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - physiology; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Plant Roots - physiology; Plants, Genetically Modified; plasma membrane; Research Paper; roots; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - physiology; Seedlings - genetics; Seedlings - metabolism; Seedlings - physiology; Sequence Analysis, DNA; suppression subtractive hybridization; tobacco; transporters; yeasts; Zea mays - genetics; Zea mays - metabolism; Zea mays - physiology; Arachis hypogaea; AhNRAMP1; Iron; Tobacco; Leguminosae; Dicotyledones; Angiospermae; Botany; Intercropping; transporter; Spermatophyta; peanut; Transport; Oil plant (vegetal); Groundnut; iron acquisition
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ers117

Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene family was isolated. Yeast complementation assays suggested that AhNRAMP1 encodes a functional iron transporter. Moreover, the mRNA level of AhNRAMP1 was obviously induced by iron deficiency in both roots and leaves. Transient expression, laser microdissection, and in situ hybridization analyses revealed that AhNRAMP1 was mainly localized on the plasma membrane of the epidermis of peanut roots. Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants.