Biosurfactant produced by novel Pseudomonas sp. WJ6 with biodegradation of n-alkanes and polycyclic aromatic hydrocarbons

• Published in: Journal of hazardous materials Vol. 276; pp. 489 - 498
• Main Authors: Xia, Wenjie, Du, Zhifeng, Cui, Qingfeng, Dong, Hao, Wang, Fuyi, He, Panqing, Tang, YongChun
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.07.2014; Elsevier
• Subjects: Alkanes - metabolism; Applied sciences; Aromatic hydrocarbons; Bacteria; Biodegradation; Biodegradation, Environmental; Degradation; Exact sciences and technology; Hazardous materials; Lipopeptides; n-Alkane; Pollution; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Pseudomonas; Pseudomonas - metabolism; Remediation; Surface-Active Agents - metabolism; Toxicity; Washing; Biodegradation; Aromatic hydrocarbons; Pseudomonas; Lipopeptides; n-Alkane; Pseudomonadales; Toxicity; HPLC chromatography; Washing; Electrospray; Persistent organic pollutant; Biosurfactant; Decontamination; Alkane; Production; Bacteria; Pseudomonadaceae; Aromatic compound; Organic compounds; Hydrocarbon; Pollutant behavior; Bioavailability; Polycyclic aromatic compound; Ionization; Mass spectrometry; Heavy oil; Structural analysis
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2014.05.062

Long chain alkanes and polycyclic aromatic hydrocarbons have been threatening the global environment because of toxicity and poor bioavailability. We isolated one novel Pseudomonas strain from heavy-oil contaminated soil which could degrade long-chain alkanes and polycyclic aromatic hydrocarbons (PAHs) and could produce the surfactin, fengycin and lichenysin, which are mostly recognized as common metabolites produced by Bacillus sp. And the heavy oil could be easily and efficiently removed from quartz sand by these lipopeptides. This paper showed that Pseudomonas is the excellent degrader of alkanes and PAHs and produce the well-known rhamnolipids and the easily neglected lipopeptides. •A novel Pseudomonas strain was isolated from heavy oil contaminated soil.•It can convert n-alkanes and polycyclic aromatic hydrocarbons to lipopeptides surfactant.•Lipopeptides surfactant have different structures when using different hydrocarbons.•Lipopetides surfactant showed great performance on heavy oil sand washing. Alkanes and polycyclic aromatic hydrocarbons (PAHs) have threatened the environment due to toxicity and poor bioavailability. Interest in degradation of these hazardous materials by biosurfactant-producing bacteria has been steadily increasing in recent years. In this work, a novel biosurfactant-producing Pseudomonas sp. WJ6 was isolated to degrade a wide range of n-alkanes and polycyclic aromatic hydrocarbons. Production of lipopeptide biosurfactant was observed in all biodegradable studies. These lipopeptides were purified and identified by C18 RP-HPLC system and electrospray ionization-mass spectrometry. Results of structural analysis showed that these lipopeptides generated from different hydrocarbons were classified to be surfactin, fengycin and lichenysin. Heavy-oil sludge washing experiments demonstrated that lipopeptides produced by Pseudomonas sp. WJ6 have 92.46% of heavy-oil washing efficiency. The obtained results indicate that this novel bacterial strain and its lipopeptides have great potentials in the environmental remediation and petroleum recovery.