Host derived inflammatory phospholipids regulate rahU (PA0122) Gene, Protein, and Biofilm Formation in Pseudomonas aeruginosa

• Published in: Cellular immunology Vol. 270; no. 2; pp. 95 - 102
• Main Authors: Rao, Jayasimha, DiGiandomenico, Antonio, Artamonov, Mykhaylo, Leitinger, Norbert, Amin, Ashok R., Goldberg, Joanna B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 2011
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - physiology; Biofilm; Biofilms - growth & development; Gene Expression; Genes, Bacterial; Host-Pathogen Interactions - genetics; Host-Pathogen Interactions - immunology; Host-Pathogen Interactions - physiology; Humans; Inflammation; Inflammation Mediators - metabolism; Models, Biological; Mutation; Oxidation-Reduction; Phospholipids; Phospholipids - metabolism; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - immunology; Pseudomonas aeruginosa - pathogenicity; Pseudomonas aeruginosa - physiology; Pseudomonas Infections - immunology; Pseudomonas Infections - microbiology; AA; PAPC; TSA; Gm; PLA2; TMB; Inflammation; Phospholipids; Pseudomonas; Gene expression; Tc; BHT; Wild type P. aeruginosa strain 383; LysoPC; CV; Asp-hemolysin; LB; Biofilm; Ox-PAPC; RahU; Mutant P. aeruginosa strain 383; Sp; Cb; BA
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2011.04.011

This study describes the role of “inflammatory” oxidized (Ox) phospholipids in regulation of rahU (PA0122) expression and biofilm formation in Pseudomonas aeruginosa (383) wild type (rahU+) and rahU mutant (rahU−) strains. Functional analysis of RahU protein from P. aeruginosa in presence of Ox-phospholipids show: (a) LysoPC modulates RahU gene/and protein expression in rahU+ cells; (b) rahU promoter activity is increased by lysoPC and inhibited by PAPC, Ox-PAPC and arachidonic acid; the latter inhibitory effect can be reversed by lysoPC, which was enzymatically derived from PAPC; (c) biofilm formation increased in rahU− cells as compared to rahU+; and (d) inhibition of rahU promoter activity by PAPC and AA (but not lysoPC) showed significantly augmented biofilm formation in rahU+ but not in rahU− cells. This study shows that host derived Ox-phospholipids affect P. aeruginosa-rahU gene and protein expression, which in turn modulates biofilm formation. The accompanying paper describes the role of RahU protein in eukaryotic-host cells.