Copper induced immunotoxicity promote differential apoptotic pathways in spleen and thymus

• Published in: Toxicology (Amsterdam) Vol. 306; pp. 74 - 84
• Main Authors: Mitra, Soham, Keswani, Tarun, Ghosh, Nabanita, Goswami, Suranjana, Datta, Anuradha, Das, Salomie, Maity, Subhajit, Bhattacharyya, Arindam
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 05.04.2013
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Apoptosis - immunology; Biocompatibility; Blotting, Western; CD4+ T cell; CD4-positive T-lymphocytes; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; CD8+ T cell; CD8-positive T-lymphocytes; CD8-Positive T-Lymphocytes - drug effects; CD8-Positive T-Lymphocytes - immunology; Cell Proliferation; Copper; Copper - immunology; Copper - toxicity; drinking water; Emergency; flow cytometry; gel electrophoresis; Gene expression; immunohistochemistry; immunotoxicity; inflammation; liver; messenger RNA; Mice; P53; Pathways; protein synthesis; Random Allocation; Reverse Transcriptase Polymerase Chain Reaction; RNA - chemistry; RNA - genetics; ROS; signal transduction; Spleen; Spleen - cytology; Spleen - drug effects; Spleen - immunology; Surgical implants; Thymus; thymus gland; Thymus Gland - cytology; Thymus Gland - drug effects; Thymus Gland - immunology; toxicology; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - immunology; Western blotting; Spleen; CD8 + T cell; ROS; CD4 + T cell; Copper; Thymus; Apoptosis; P53; CD4+ T cell; CD8+ T cell
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2013.01.001

Graphical abstract Highlights ► Copper-induced ROS generation and mitochondrial trans-membrane potential changes result in different consequences in spleen and thymus. ► Inflammation appeared in both the spleen and thymus after to copper treatment. ► Apoptosis in the spleen appears to follow a p53-independent pathway. ► Apoptosis in the thymus appears to follow a p53-dependent intrinsic and extrinsic pathway. ► In both the spleen and thymus, the CD4+ T cell population decreased and CD8+ T cell population increased after copper treatment.