SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells

• Published in: Journal of oral pathology & medicine Vol. 31; no. 6; pp. 345 - 352
• Main Authors: Fujita, Tsuyoshi, Shiba, Hideki, Sakata, Masatoshi, Uchida, Yuushi, Nakamura, Shigeo, Kurihara, Hidemi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science, Ltd 01.07.2002; Blackwell
• Subjects: Alkaline Phosphatase - metabolism; ALPase activity; Analysis of Variance; Biological and medical sciences; Blotting, Northern; Blotting, Western; Bone Remodeling; Cell Count; Cells, Cultured; Degeneration. Regeneration. Wound healing. Graft; Dentistry; DNA - biosynthesis; Fundamental and applied biological sciences. Psychology; Glycoproteins - biosynthesis; human periodontal ligament cells; Humans; Matrix Metalloproteinase 2 - biosynthesis; MMP-2; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus; OCIF; OPG; Osteonectin - metabolism; Osteoprotegerin; Periodontal Ligament - cytology; Periodontal Ligament - metabolism; Receptors, Cytoplasmic and Nuclear - biosynthesis; Receptors, Tumor Necrosis Factor; Regeneration; RNA, Messenger - analysis; SPARC; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vertebrates: digestive system; Cell culture; Periodontal ligament; Enzyme; Parodontium; Extracellular matrix; DNA synthesis; Osteoclast; Physiology; Experimental study; Cicatrization; Formation; Metalloproteins
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1034/j.1600-0714.2002.00188.x

Background:  Osteonectin/secreted protein, acidic and rich in cysteine (SPARC) is expressed in periodontal ligaments. Therefore, a better understanding of the action of SPARC on periodontal ligament cells could help to elucidate remodelling and repair mechanisms in periodontal tissue. In the present study, we examined the effects of human platelet‐derived SPARC (hp‐SPARC) on the expressions of SPARC and osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF), alkaline phosphatase (ALPase) activity, matrix metalloproteinase‐2 (MMP‐2) production and DNA synthesis in cultures of human periodontal ligament (HPL) cells. Methods:  HPL cells at the sixth passage were exposed to hp‐SPARC. The expression of OPG/OCIF and SPARC mRNAs was examined by Northern blot analysis. The protein levels for OPG/OCIF and MMP‐2 were determined by Western blot analysis. ALPase activity was measured by the method of Bessey et al. DNA synthesis was estimated by incorporation of [3H]thymidine. Results:  Hp‐SPARC enhanced OPG/OCIF synthesis at the protein and mRNA levels. Hp‐SPARC also enhanced DNA and MMP‐2 synthesis dose‐dependently, but had little effect on ALPase activity and SPARC mRNA expression. Conclusion:  SPARC may play a role in remodelling and repair of periodontal tissue by promoting proliferation and MMP‐2 production. It may also regulate osteoclast formation through OPG/OCIF in periodontal ligament cells.