Molded bone augmentation by a combination of barrier membrane and recombinant human bone morphogenetic protein-2

• Published in: Oral diseases Vol. 7; no. 5; pp. 281 - 286
• Main Authors: Takeishi, H, Irie, K, Okuda, K, Ozawa, H, Yajima, T, Ejiri, S
• Format: Journal Article
• Language: English
• Published: Copenhagen K, Denmark Munksgaard International Publishers 01.09.2001; Blackwell
• Subjects: alkaline phosphatase; Animals; barrier membrane; Biological and medical sciences; bone augmentation; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins - pharmacology; Dentistry; Guided Tissue Regeneration - methods; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Humans; Immunoenzyme Techniques; Male; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Membranes, Artificial; Neurosurgery; Osteoblasts - drug effects; Osteoblasts - enzymology; Osteogenesis - drug effects; Polytetrafluoroethylene; Rats; Rats, Wistar; recombinant human bone morphogenetic protein-2 (rhBMP-2); Recombinant Proteins - pharmacology; Skull; Skull, brain, vascular surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; tartrate-resistant acid phosphatase; Tissue Engineering - methods; Transforming Growth Factor beta; Rat; Rodentia; Maxillary; Increase; Barrier; Experimental study; Premises; Plasty; Vertebrata; Calvaria; Mammalia; Bone morphogenetic protein; Treatment; Surgery; Animal; Membrane; Technique; Face
• ISSN: 1354-523X; 1601-0825
• DOI: 10.1034/j.1601-0825.2001.00725.x

OBJECTIVES: To provide the histological background to a new method of local bone augmentation, we examined the events occurring beneath a barrier membrane applied with recombinant human bone morphogenetic protein‐2 (rhBMP‐2). MATERIALS AND METHODS: The effects on bone augmentation of rhBMP‐2, applied with a membrane mold (BMP‐Memb), over surgically‐induced bone defects in rat calvaria were examined histologically, and the results compared with those from application of rhBMP‐2 (BMP) alone, or of a molded membrane (Memb) alone. RESULTS: At postoperative week 2, the BMP group showed the most marked bone formation. However, the bone diminished in size by week 8. The Memb group showed slow but continuous bone formation by week 8. In the BMP‐Memb group, bone filled the space in the mold at week 2, and this was maintained until week 8. Moreover, the soft tissue that had intervened between newly formed bone and the membrane in the Memb group was not evident in the BMP‐Memb group, in which bone had formed directly on the membrane. CONCLUSIONS: The results suggest that the combination of rhBMP‐2 and barrier membrane has advantages in producing and maintaining bone in the intended shape by inducing osteoblasts directly on the inner surface of the membrane.