Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge

• Published in: Journal of Clinical Microbiology Vol. 39; no. 5; pp. 1903 - 1911
• Main Authors: SCHROEDER, Jill M, BOOTON, Gregory C, HAY, John, NISZL, Ingrid A, SEAL, David V, MARKUS, Miles B, FUERST, Paul A, BYERS, Thomas J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2001
• Subjects: Acanthamoeba; Acanthamoeba - classification; Acanthamoeba - genetics; Acanthamoeba - isolation & purification; Acanthamoeba Keratitis - parasitology; Amibiasis; Animals; Biological and medical sciences; Cornea - parasitology; DNA Primers; DNA, Protozoan - analysis; DNA, Protozoan - genetics; DNA, Ribosomal - analysis; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; General aspects and techniques; Genotype; Human protozoal diseases; Humans; Infectious diseases; Medical sciences; Molecular Sequence Data; Parasitic diseases; Parasitology; Phylogeny; Polymerase Chain Reaction - methods; Protozoa; Protozoal diseases; RNA, Ribosomal, 18S - genetics; rRNA 18S; Sensitivity and Specificity; Sequence Analysis, DNA; Sewage - parasitology; Human; Protozoa; Protozoal disease; Lobosea; Typing; Nucleotide sequence; Ribosomal DNA; Genotype; Genus specificity; Identification; Parasitosis; 18S-RNA; Infection; Polymerase chain reaction; Acanthamoeba; Amebiasis; Diagnosis

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