Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge

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Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 39; no. 5; pp. 1903 - 1911
Main Authors SCHROEDER, Jill M, BOOTON, Gregory C, HAY, John, NISZL, Ingrid A, SEAL, David V, MARKUS, Miles B, FUERST, Paul A, BYERS, Thomas J
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.05.2001
Subjects
Acanthamoeba
Acanthamoeba - classification
Acanthamoeba - genetics
Acanthamoeba - isolation & purification
Acanthamoeba Keratitis - parasitology
Amibiasis
Animals
Biological and medical sciences
Cornea - parasitology
DNA Primers
DNA, Protozoan - analysis
DNA, Protozoan - genetics
DNA, Ribosomal - analysis
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Genotype
Human protozoal diseases
Humans
Infectious diseases
Medical sciences
Molecular Sequence Data
Parasitic diseases
Parasitology
Phylogeny
Polymerase Chain Reaction - methods
Protozoa
Protozoal diseases
RNA, Ribosomal, 18S - genetics
rRNA 18S
Sensitivity and Specificity
Sequence Analysis, DNA
Sewage - parasitology
Human
Protozoa
Protozoal disease
Lobosea
Typing
Nucleotide sequence
Ribosomal DNA
Genotype
Genus specificity
Identification
Parasitosis
18S-RNA
Infection
Polymerase chain reaction
Acanthamoeba
Amebiasis
Diagnosis
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content type line 23
Present address: 336 Glagow Rd., Ralston, Paisley, PA1 3BH, Scotland, United Kingdom.
Present address: Department of Optometry and Vision Science, City University, London EC1 V7DD, England.
Corresponding author. Mailing address: Department of Molecular Genetics, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210-1292. Phone: (614) 292-5963. Fax: (614) 292-4466. E-mail: byers.2@osu.edu.
Present address: Department of Biochemistry, Indiana University School of Medicine, Indianapolis, IN 46202.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.39.5.1903-1911.2001