Interactions of TRIC agents with macrophages and BHK-21 cells observed by electron microscopy

TRIC agents do not multiply in mouse peritoneal macrophages in culture but have a toxic effect on them, whereas they multiply readily in BHK-21 cells. Sections of macrophages and of BHK-21 cells fixed during the first 90 min after inoculation were examined by electron microscopy. Macrophages ingeste...

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Bibliographic Details
Published inThe Journal of hygiene Vol. 71; no. 3; pp. 515 - 528
Main Authors Lawn, A. M., Blyth, W. A., Taverne, Janice
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.09.1973
Subjects
Acid Phosphatase - analysis
Animals
Cell Line
Cell Membrane
Chlamydia - isolation & purification
Conjunctivitis, Inclusion - microbiology
Cricetinae
Cytoplasm
In Vitro Techniques
Inoculation
Inoculum
Kidney
L cells
Latex
Lawns
Lysosomes
Macrophages
Mice
Microscopy, Electron
Phagocytosis
Time Factors
Trachoma - microbiology
Vacuoles
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Summary:TRIC agents do not multiply in mouse peritoneal macrophages in culture but have a toxic effect on them, whereas they multiply readily in BHK-21 cells. Sections of macrophages and of BHK-21 cells fixed during the first 90 min after inoculation were examined by electron microscopy. Macrophages ingested all forms of the organism, which were eventually degraded in lysosomes. However, elementary bodies were distinguished from other TRIC particles by the delay in their transfer to lysosomes. BHK-21 cells ingested elementary bodies selectively, and in these cells the organisms were neither found in lysosomes nor degraded. Instead they showed morphological changes that probably represented an early stage of development.
Bibliography:PII:S0022172400046507
istex:B4F10D9C80FFB59E61AF0E44458693319B031F9D
ArticleID:04650
Medical Research Council Trachoma Unit
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ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1724
2396-8184
DOI:10.1017/S0022172400046507