Protective effect of Spirulina platensis enriched in phenolic compounds against hepatotoxicity induced by CCl4

• Published in: Food chemistry Vol. 141; no. 3; pp. 1972 - 1979
• Main Authors: Kepekçi, Remziye Aysun, Polat, Sait, Çelik, Ahmet, Bayat, Nuray, Saygideger, Saadet Demirörs
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Alanine Transaminase - metabolism; Animals; Antioxidant activity; Antioxidants - metabolism; Aspartate Aminotransferases - metabolism; Biological and medical sciences; Carbon Tetrachloride - toxicity; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - enzymology; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Food toxicology; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Hepatoprotectivity; Humans; Liver - drug effects; Liver - enzymology; Liver - metabolism; Male; Malondialdehyde - metabolism; Medical sciences; Phenolic compounds; Phenols - administration & dosage; Protective Agents - administration & dosage; Rats; Rats, Wistar; Secondary metabolites; Spirulina - chemistry; Spirulina platensis; Superoxide Dismutase - metabolism; Toxicology; Hepatoprotectivity; Secondary metabolites; Phenolic compounds; Spirulina platensis; Secondary; Metabolite; Toxicity; Liver; Carbon tetrachloride; Antioxidant activity; Hepatic disease; Antioxidant; Prevention; Cyanobacteria; Phenols; Bacteria; Digestive diseases
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.04.107

•The hepatoprotective effect of Spirulina platensis enriched in phenolics (SP2) was examined.•SP2 was more potent than S. platensis with low amounts of phenolics (SP1).•The CCl4-induced increases in ALT and AST were attenuated by SP2.•The hepatic lessions were counteracted more effectively by SP2 pretreatment.•SP2 pretreatment also showed more improvement in ultrastructure of livers of rats. Phenolic compounds make up the major secondary metabolites with high pharmaceutical potential. Microalgae were reported to contain low amounts of phenolic compounds. The present study aimed to investigate the hepatoprotective potential of biomass of Spirulina platensis enriched in phenolic compounds. The protective effects of the biomass of S. platensis with low amounts of phenolics (SP1) and with high amounts of phenolics (SP2) against CCl4-induced acute hepatotoxicity were evaluated in rats. The increased levels of ALT, AST and MDA along with decreased activities of SOD and CAT were significantly (p<0.01) ameliorated by SP2. Histological examinations revealed that SP2 was more potent than SP1 in protecting the liver from toxic injury of CCl4 and preserving the hepatocyte ultrastructure. The lesions including necrosis, lymphocyte infiltration, ballooning degeneration and hepatocyte injury as irregular lamellar organisation, dilations in endoplasmic reticulums and the presence of great number of cytoplasmic vacuolization were healed by SP2.