TrgI, toluene repressed gene I, a novel gene involved in toluene-tolerance in Pseudomonas putida S12

• Published in: Extremophiles : life under extreme conditions Vol. 13; no. 2; pp. 283 - 297
• Main Authors: Volkers, Rita J. M, Ballerstedt, Hendrik, Ruijssenaars, Harald, de Bont, Jan A. M, de Winde, Johannes H, Wery, Jan
• Format: Journal Article
• Language: English
• Published: Japan Japan : Springer Japan 01.03.2009; Springer Japan; Springer; Springer Nature B.V
• Subjects: Bacteria; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacteriology; Biochemistry; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biomedical and Life Sciences; Biotechnology; Energy demand; Enzymes; Flagella - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - drug effects; Genes; Genes, Bacterial; Genetics; Hydrocarbons; Life Sciences; Microbial Ecology; Microbiology; Mission oriented research; Oligonucleotide Array Sequence Analysis; Original Paper; Polymerase Chain Reaction; Principal Component Analysis; Pseudomonas putida; Pseudomonas putida - metabolism; Solvents; Solvents - chemistry; Time Factors; Toluene; Toluene - chemistry; Transcription, Genetic; Solvent-tolerance; S12; Transcriptomics; Toluene; Pseudomonadales; Tolerance; Pollutant; Organic solvent; Pseudomonas putida S12; Gene; trgI; Bacteria; Pseudomonadaceae; Extreme environment; Pseudomonas putida
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s00792-008-0216-0

Pseudomonas putida S12 is well known for its remarkable solvent tolerance. Transcriptomics analysis of this bacterium grown in toluene-containing chemostats revealed the differential expression of 253 genes. As expected, the genes encoding one of the major solvent tolerance mechanisms, the solvent efflux pump SrpABC and its regulatory genes srpRS were heavily up-regulated. The increased energy demand brought about by toluene stress was also reflected in transcriptional changes: genes involved in sugar storage were down-regulated whereas genes involved in energy generation such as isocitrate dehydrogenase and NADH dehydrogenases, were up-regulated in the presence of toluene. Several flagella-related genes were up-regulated and a large group of transport genes were down-regulated. In addition, a novel Pseudomonas-specific gene was identified to be involved in toluene tolerance of P. putida S12. This toluene-repressed gene, trgI, was heavily down-regulated immediately upon toluene exposure in batch cultures. The relationship of trgI with solvent tolerance was confirmed by the increased resistance to toluene shock and toluene induced lysis of trgI knock-out mutants. We propose that down-regulation of trgI plays a role in the first line of defence against solvents.