Effect of legume seed extracts on the inhibition of proteolytic activity and muscle degradation of fresh water prawn (Macrobrachiumrosenbergii)

• Published in: Food chemistry Vol. 129; no. 3; pp. 1093 - 1099
• Main Authors: Sriket, Chodsana, Benjakul, Soottawat, Visessanguan, Wonnop, Hara, Kenji
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2011; Elsevier
• Subjects: Arachis hypogaea; Biological and medical sciences; Collagenase; Degradation; Fish and seafood industries; Food industries; Fresh water prawn; Freshwater; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Glycine max; Legume seed; Macrobrachium rosenbergii; Muscle proteins; Protease; Trypsin inhibitor; Vigna angularis; Vigna subterranea; Degradation; Trypsin inhibitor; Protease; Legume seed; Collagenase; Muscle proteins; Fresh water prawn; Seeds; Fresh water; Extract; Macrura; Crustacea; Proteolysis; Muscle; Decapoda; Inhibition; Shrimp; Serine endopeptidases; Enzyme; Protein; Peptidases; Grain legume; Edible crustacean; Trypsin; Arthropoda; Hydrolases; Inhibitor; Invertebrata
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2011.05.080

► Extracts from soybean and bambara groundnut contained high trypsin inhibitors. ► Those extracts were able to inhibit hepatopancreas proteases (HP) effectively. ► Degradation of muscle proteins caused by HP was suppressed by those extracts. Trypsin inhibitors in the extracts from soybean (Glycine max), adzuki bean (Vigna angularis), bambara groundnut (Vigna subterranea) and red kidney bean (Phaseoulus vulgaris) varied in amount and molecular weight. The soybean extract had the highest level of trypsin inhibitor with molecular weight (MW) of 21kDa, followed by bambara groundnut extract possessing trypsin inhibitor with MW of 15kDa. Both extracts showed a more effective inhibition towards crude protease extract (CE) from the hepatopancreas of fresh water prawn (Macrobrachium rosenbergii) than the extracts from adzuki and red kidney beans. Activity staining also reconfirmed the higher inhibitory activity on CE from hepatopancreas by the extracts from both soybean and bambara groundnut. The extracts from all seeds were able to inhibit the degradation of fresh water prawn meat containing CE in a concentration dependent manner. Based on inhibitor study, the extracts from soybean and bambara groundnut can be a potential aid to suppress the muscle softening of fresh water prawn, mediated by trypsin-like proteases released from hepatopancreas, during extended iced storage.