Time-course alterations of gut microbiota and short-chain fatty acids after short-term lincomycin exposure in young swine

• Published in: Applied microbiology and biotechnology Vol. 105; no. 21-22; pp. 8441 - 8456
• Main Authors: Tang, Shanlong, Zhang, Shunfen, Zhong, Ruqing, Su, Dan, Xia, Bing, Liu, Lei, Chen, Liang, Zhang, Hongfu
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2021; Springer; Springer Nature B.V
• Subjects: Acid production; Antibacterial agents; Antibiotics; Applied Microbial and Cell Physiology; Bacteria; Biomedical and Life Sciences; Biotechnology; Body weight; Coliforms; Digestive system; Disruption; Dosage and administration; Drug therapy; Dysbacteriosis; Escherichia; Exposure; Fatty acids; Gastrointestinal tract; Hogs; Homeostasis; Immunoregulation; Intestinal microflora; Lactobacillus; Life Sciences; Lincomycin; Microbial Genetics and Genomics; Microbiology; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Pathogens; Patient outcomes; Physiological aspects; Probiotics; Relative abundance; Shigella; Swine; China; Gut dysfunction; Short-chain fatty acid; Young pigs; Fecal microbiota; Antibiotic exposure
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11627-x

Increasing evidence suggests that antibiotic administration causes gut injury, negatively affecting nutrient digestion, immune regulation, and colonization resistance against pathogens due to the disruption of gut microbiota. However, the time-course effects of therapeutic antibiotics on alterations of gut microbes and short-chain fatty acids (SCFAs) in young swine are still unknown. In this study, twenty piglets were assigned into two groups and fed commercial diets with or without lincomycin in the first week for a 28-day trial period. Results showed that 1-week lincomycin exposure (LE) did reduce the body weight on day 14 ( p  = 0.0450) and 28 ( p  = 0.0362). The alpha-diversity notably reduced after 1-week LE, and then gradually raised and reached the control group level in the second week on cessation of LE, indicated by the variation of Sobs, Chao, Shannon, and ACE index ( p  < 0.05). Beta-diversity analysis revealed that the distinct microbial cluster existed persistently for the whole trial period between two groups ( p  < 0.001). The relative abundance of most microbes including fiber-degrading (e.g., Agathobacter and Coprococcus ), beneficial (e.g., Lactobacillus and Mitsuokella ), or pathogenic bacteria (e.g., Terrisporobacter and Lachnoclostridium ) decreased (LDA score > 3), and the concentration of SCFAs also diminished in the feces of 1-week lincomycin-administrated young swine, indicating that therapeutic LE killed most bacteria and reduced SCFA production with gut dysbiosis occurring. After the LE stopped, the state of gut dysbiosis gradually attenuated and formed new gut-microbe homeostasis distinct from microbial homeostasis of young pigs unexposed to lincomycin. The increased presence of potential pathogens, such as Terrisporobacter , Negativibacillus , and Escherichia-Shigella , and decreased beneficial bacteria, such as Lactobacillus and Agathobacter , were observed in new homeostasis reshaped by short-lincomycin administration (LDA score > 3 or p  < 0.05), adversely affecting gut development and health of young pigs. Collectively, these results suggested that severe disruption of the commensal microbiota occurred after short-term LE or termination of LE in young swine. Key points • Therapeutic lincomycin exposure induced gut dysbiosis, killing most bacteria and reducing short-chain fatty acid production. • Gut dysbiosis gradually attenuated and formed new homeostasis after lincomycin exposure stopped. • The new homeostasis, increased Escherichia-Shigella etc. and decreased Lactobacillus etc., was potentially harmful to gut health.