A diallel study to detect genetic background variation for FHB resistance in winter wheat

• Published in: Scientific reports Vol. 14; no. 1; p. 4614
• Main Authors: Neupane, Bipin, Bisek, Bradley, Marais, Francois
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 26.02.2024; Nature Publishing Group UK; Nature Portfolio
• Subjects: Chromosome Mapping; Combining ability; Disease resistance; Disease Resistance - genetics; Epidemics; Fusarium - genetics; Fusarium head blight; Genes; Genetic diversity; Genotypes; Greenhouses; Hybrids; Pathogens; Plant Breeding; Plant Diseases - genetics; Quantitative Trait Loci; Triticum - genetics; Variance analysis; Wheat; Winter wheat
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-53710-z

Breeding for resistance to Fusarium head blight (FHB) relies strongly on a limited number of larger-effect resistance QTL that have been mapped and associated with nearby markers. Smaller-effect (background) resistance QTL may also contribute moderate levels of resistance yet are mostly poorly characterized. Overall resistance of a genotype is determined by the combined action of both types of resistance QTL. This study aimed to identify well-adapted, advanced hard red winter (HRW) wheat breeding lines with useful background resistance QTL. A diallel trial consisting of 11 parents and 55 non-reciprocal F hybrids was tested for Type II FHB resistance in a replicated greenhouse experiment. Significant differences were detected among entries for disease severity (DS), general combining ability (GCA) and specific combining ability (SCA) with four parents being identified as the best general combiners with lowest DS. The ratio of GCA:SCA effects suggested that additive QTL effects were of primary importance. Overall, resistance QTL showed incomplete dominance, an excess of dominant alleles, and a greater contribution of positive effect genes. F of the six best F hybrids with the lowest DS were compared in a second greenhouse FHB trial to select possible transgressive segregates for continued evaluation and line development.