Radical cation scavenging activity of berberine bridge enzyme-like oligosaccharide oxidases acting on short cell wall fragments

• Published in: Scientific reports Vol. 13; no. 1; p. 4123
• Main Authors: Scortica, Anna, Scafati, Valentina, Giovannoni, Moira, Benedetti, Manuel, Mattei, Benedetta
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 13.03.2023; Nature Publishing Group UK; Nature Portfolio
• Subjects: Apoplast; Berberine; Cations - metabolism; Cell Wall - metabolism; Cell walls; Cellulose; Enzymes; Homeostasis; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Laccase - metabolism; Oligogalacturonides; Oligomers; Oligosaccharides; Oligosaccharides - metabolism; Oligosaccharides - pharmacology; Oxidation-Reduction; Oxidative stress; Polymerization
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-31335-y

Oligogalacturonide-oxidases (OGOXs) and cellodextrin-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide-oxidases (OSOXs) that oxidize, respectively, oligogalacturonides (OGs) and cellodextrins (CDs), thereby inactivating their elicitor nature and concomitantly releasing H O . Little is known about the physiological role of OSOX activity. By using an ABTS -reduction assay, we identified a novel reaction mechanism through which the activity of OSOXs on cell wall oligosaccharides scavenged the radical cation ABTS with an efficiency dependent on the type and length of the oxidized oligosaccharide. In contrast to the oxidation of longer oligomers such as OGs (degree of polymerization from 10 to 15), the activity of OSOXs on short galacturonan- and cellulose-oligomers (degree of polymerization ≤ 4) successfully counteracted the radical cation-generating activity of a fungal laccase, suggesting that OSOXs can generate radical cation scavenging activity in the apoplast with a power proportional to the extent of degradation of the plant cell wall, with possible implications for redox homeostasis and defense against oxidative stress.