Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses

• Published in: Virologica Sinica Vol. 31; no. 1; pp. 49 - 56
• Main Authors: Wang, Jingjing, Deng, Feng, Ye, Gang, Dong, Wanyu, Zheng, Anjun, He, Qigai, Peng, Guiqing
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.02.2016; KeAi Publishing Communications Ltd
• Subjects: Amino Acid Sequence; Animals; Antiviral Agents; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Line; Comparative analysis; Coronaviridae; Coronavirus - genetics; Coronavirus - metabolism; Coronavirus - physiology; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Coronaviruses; Diarrhea; Drug Evaluation, Preclinical; Drug screening; Gastroenteritis; Glycoproteins; Haplorhini; Humans; Kidneys; Lentivirus; Lentivirus - genetics; Lentivirus - metabolism; Lentivirus - physiology; Lentivirus Infections - veterinary; Lentivirus Infections - virology; Medical Microbiology; Membrane Glycoproteins; Microbial Genetics and Genomics; Microbiology; Oncology; Proteins; Receptors, Virus - metabolism; Research Article; Sequence Alignment; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - metabolism; Spike Glycoprotein, Coronavirus - physiology; Swine; Swine Diseases - virology; Transmissible gastroenteritis; Transmissible gastroenteritis virus; Viral Tropism; Virology; Virus Internalization; Viruses; 临床; 医学; 病毒学; 药物学; cell entry; receptor binding; Coronavirus; pseudotyped virus; spike proteins
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-015-3690-4

The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins（S） were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus（PEDV） and transmissible gastroenteritis virus（TGEV） have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81（monkey kidney）, Huh-7（human liver）, and PK-15（pig kidney） cells efficiently. CCL94（cat kidney） cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.