DNA amplification using phi29 DNA polymerase validates gene polymorphism analysis from buccal mucosa samples

Venous blood is currently the most common source of DNA for gene polymorphism screening; however, blood sampling is invasive and difficult to perform in general dental treatment. Buccal mucosa samples provide an alternative source of DNA, but it is frequently difficult to effectively amplify the DNA...

Full description

Saved in:
Bibliographic Details
Published inJournal of prosthodontic research Vol. 55; no. 3; pp. 165 - 170
Main Authors Taniguchi, R., Masaki, C., Murashima, Y., Makino, M., Kojo, T., Nakamoto, T., Hosokawa, R.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.07.2011
Subjects
Buccal mucosa
DNA amplification
DNA-Directed DNA Polymerase - analysis
Gene polymorphism
Humans
Interleukin-1 - genetics
Mouth Mucosa - chemistry
Nucleic Acid Amplification Techniques - methods
Peri-implantitis
Polymorphism, Genetic
Viral Proteins
Buccal mucosa
Peri-implantitis
DNA amplification
Gene polymorphism
Online AccessGet full text

Cover

More Information
Summary:Venous blood is currently the most common source of DNA for gene polymorphism screening; however, blood sampling is invasive and difficult to perform in general dental treatment. Buccal mucosa samples provide an alternative source of DNA, but it is frequently difficult to effectively amplify the DNA owing to the small amounts of sample material obtained. This study was performed to establish a method for performing total genomic DNA amplification from buccal mucosa samples using phi29 DNA polymerase. Total genomic DNA was isolated from buccal mucosa samples obtained from healthy subjects and was amplified using phi29 DNA polymerase. To determine the suitability of the extracted DNA for genotyping, polymerase chain reaction and restriction fragment length polymorphism analyses were performed for the IL-1 gene polymorphism. Genotyping of the IL-1 polymorphism was successful using the amplified DNA from a buccal mucosa, but genotyping was unsuccessful using the unamplified control because of low DNA purity. The method of extracting DNA from a buccal mucosa is painless, simple, minimally invasive, and rapid. Genomic DNA from a buccal mucosa can be amplified by phi29 DNA polymerase in sufficient quantity and quality to conduct gene polymorphism analyses.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1883-1958
2212-4632
2212-4632
DOI:10.1016/j.jpor.2010.12.001