Sequence-specific recognition of methylated DNA by human zinc-finger proteins

DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achi...

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Bibliographic Details
Published inNucleic acids research Vol. 38; no. 15; pp. 5015 - 5022
Main Authors Sasai, Nobuhiro, Nakao, Mitsuyoshi, Defossez, Pierre-Antoine
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.08.2010
Subjects
Base Sequence
Binding Sites
Consensus Sequence
DNA Methylation
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Gene Regulation, Chromatin and Epigenetics
Humans
Protein Binding
Repressor Proteins - chemistry
Repressor Proteins - metabolism
Transcription Factors - metabolism
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Summary:DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkq280