Cytokine-Induced Monocyte Characteristics in SLE

Monocytes in SLE have been described as having aberrant behavior in a number of assays. We examined gene expression and used a genome-wide approach to study the posttranslational histone mark, H4 acetylation, to examine epigenetic changes in SLE monocytes. We compared SLE monocyte gene expression an...

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Bibliographic Details
Published inBioMed research international Vol. 2010; no. 2010; pp. 1 - 13
Main Authors Song, Li, Perin, Juan C., Maurer, Kelly, Zhang, Zhe, Sullivan, Kathleen E.
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2010
Hindawi Limited
Subjects
Acetylation - drug effects
Apoptosis
Biomarkers - metabolism
Biomedical research
Cell Membrane - drug effects
Cell Membrane - metabolism
Cluster Analysis
Cytokines - pharmacology
Data processing
Deoxyribonucleic acid
DNA
Experiments
Flow cytometry
Gene Expression Regulation - drug effects
Histones - metabolism
Humans
Interferon
Interferons - pharmacology
Lupus
Lupus Erythematosus, Systemic - genetics
Lupus Erythematosus, Systemic - immunology
Lupus Erythematosus, Systemic - pathology
Monocytes - drug effects
Monocytes - immunology
Promoter Regions, Genetic - genetics
Statistical methods
Studies
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Summary:Monocytes in SLE have been described as having aberrant behavior in a number of assays. We examined gene expression and used a genome-wide approach to study the posttranslational histone mark, H4 acetylation, to examine epigenetic changes in SLE monocytes. We compared SLE monocyte gene expression and H4 acetylation with three types of cytokine-treated monocytes to understand which cytokine effects predominated in SLE monocytes. We found that γ-interferon and α-interferon both replicated a broad range of the gene expression changes seen in SLE monocytes. H4 acetylation in SLE monocytes was overall higher than in controls and there was less correlation of H4ac with cytokine-treated cells than when gene expression was compared. A set of chemokine genes had downregulated expression and H4ac. Therefore, there are significant clusters of aberrantly expressed genes in SLE which are strongly associated with altered H4ac, suggesting that these cells have experienced durable changes to their epigenome.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Academic Editor: George C. Tsokos
ISSN:2314-6133
1110-7243
2314-6141
1110-7251
DOI:10.1155/2010/507475