Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

• Published in: World journal of microbiology & biotechnology Vol. 25; no. 4; pp. 721 - 725
• Main Authors: Cui, Fengjie, Li, Yin, Liu, Zhiqiang, Zhao, Hui, Ping, Lifeng, Ping, Liying, Yang, Yinan, Xue, Yaping, Yan, Lijiao
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.04.2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Applied Microbiology; Biochemistry; Biological and medical sciences; Biomass energy; Biomedical and Life Sciences; Biotechnology; Design optimization; Environmental Engineering/Biotechnology; Enzymes; Fermentation; Fundamental and applied biological sciences. Psychology; Fungi; Independent variables; Life Sciences; Methods; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; Morphology; Optimization; Original Paper; Penicillium; Science; Studies; Variables; ZH-19; Identification; Response surface methodology; Xylanase; Optimization; Enzyme; Penicillium; Endo-1,4-β-xylanase; Fermentation; Fungi; Glycosylases; Statistical method; Glycosidases; Production; Hydrolases; Response surface; Xylan endo-1,3-β-xylosidase; Fungi Imperfecti; Penicillium thiersii ZH-19
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-008-9942-9

The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8°C, xylan 13.2 g l⁻¹ and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml⁻¹. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL⁻¹ in the flask experiments and 80.23 U mL⁻¹ in the scale of 15-L fermenter under the optimal condition.