Decolorization of reactive dyes by Clostridium bifermentans SL186 isolated from contaminated soil

• Published in: World journal of microbiology & biotechnology Vol. 24; no. 10; pp. 2221 - 2226
• Main Authors: Joe, Min-Ho, Lim, Sang-Young, Kim, Dong-Ho, Lee, In-Soo
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.10.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Amines; Anaerobic conditions; Applied Microbiology; Azo dyes; Bacteria; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Clostridium bifermentans; Dyes; Effluents; Environmental Engineering/Biotechnology; Enzymes; Fundamental and applied biological sciences. Psychology; Glucose; Industrial effluents; Industrial wastewater; Life Sciences; Microbiology; Original Paper; Soil contaminants; Soil contamination; Studies; Textile industry; Reactive azo dye; Decolorization; Industrial effluent; Discoloration; Clostridium bifermentans; Azo dye; Clostridiaceae; Clostridiales; Bacteria; Soil pollution; Effluent
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-008-9733-3

Decolorization of textile reactive azo dyes by a strain of bacteria (SL186) isolated from a contaminated site was investigated. SL186 was identified as Clostridium bifermentans by phenotypic characterization and 16S rDNA sequence comparison. Under anaerobic conditions, SL186 had decolorized the dyes Reactive Red 3B-A, Reactive Black 5, and Reactive Yellow 3G-P by over 90% after 36 h post-inoculation. The bacterium retained decolorizing activity over a wide range of pH values (6-12), with peak activity at pH 10. Additionally, SL186 decolorized a relatively high concentration of Reactive Red 3B-A dye (1,000 ppm) by over 80% and raw industrial effluent effectively. The addition of glucose increased the decolorization rate a little. Spectrophotometric analyses of the reactive dyes showed no distinct peak indicating aromatic amines. However, a new peak was detected between 300 and 450 nm from the decolorized raw industrial effluent. These results suggest that C. bifermentans SL186 is a suitable bacterium for the biological processing of dye-containing wastewater.