Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14

The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete mole...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 107; no. 11; pp. 4919 - 4924
Main Authors Koopman, Frank, Wierckx, Nick, de Winde, Johannes H., Ruijssenaars, Harald J., Ingram, Lonnie O'Neal
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 16.03.2010
National Acad Sciences
Subjects
Alcohols
Aldehydes
Bacteria
Biochemical pathways
Biochemistry
Biological Sciences
Carbon
Cell extracts
Cupriavidus - cytology
Cupriavidus - genetics
Cupriavidus - growth & development
Cupriavidus - metabolism
Dehydrogenases
DNA Transposable Elements - genetics
Enzymes
Furaldehyde - analogs & derivatives
Furaldehyde - metabolism
Genes
Genes, Bacterial - genetics
Genetics
Metabolic Networks and Pathways
Metabolism
Molecules
Mutagenesis - genetics
Oxidoreductases - metabolism
Phenotype
Pseudomonas putida
Pseudomonas putida - metabolism
Studies
Transposons
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Summary:The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
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1F.K. and N.W. contributed equally to this work.
3Present address: BIRD Engineering BV, Westfrankelandsedijk 1, 3115 HG Schiedam, The Netherlands.
Edited* by Lonnie O’Neal Ingram, University of Florida, Gainesville, Gainesville, FL, and approved January 19, 2010 (received for review November 11, 2009)
Author contributions: J.H.d.W. and H.J.R. designed research; F.K. and N.W. performed research; F.K. and N.W. analyzed data; and F.K., N.W., J.H.d.W., and H.J.R., wrote the paper.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0913039107