Acute effects of lactic acid-fermented and enzyme-digested soybean on protein synthesis via mTOR signaling in the skeletal muscle

• Published in: Bioscience, biotechnology, and biochemistry Vol. 84; no. 11; pp. 2360 - 2366
• Main Authors: Ono, Kohei, Tanaka, Minoru, Ikeji, Takuya, Nakanishi, Ryosuke, Hirabayashi, Takumi, Tategaki, Airo, Kondo, Hiroyo, Ishihara, Akihiko, Fujino, Hidemi
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.11.2020; Oxford University Press
• Subjects: Animals; Digestion; Lactic Acid - metabolism; lactic acid-fermented and enzyme-digested soybean; Male; Mice; Mice, Inbred ICR; mTOR pathway; muscle protein synthesis; Muscle, Skeletal - anatomy & histology; Muscle, Skeletal - cytology; Muscle, Skeletal - drug effects; Organ Size - drug effects; Peptide Hydrolases - metabolism; ribosomal protein S6; Signal Transduction - drug effects; Soybean; Soybean Proteins - metabolism; Soybean Proteins - pharmacology; TOR Serine-Threonine Kinases - metabolism; muscle protein synthesis; mTOR pathway; Soybean; ribosomal protein S6; lactic acid-fermented and enzyme-digested soybean
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2020.1795810

Protein-containing nutrients result in the efficient hypertrophy of muscles by increasing muscle protein synthesis. Soybean is often ingested by athletes or individuals who exercise; however, it takes very long to be absorbed. Lactic acid-fermented and enzyme-digested (LFED) soybean is absorbed faster than untreated soybean. This study aims at determining muscle protein synthesis after ingesting a single bolus of soybean or LFED soybean produced by lactic acid bacteria and protease digestion. Eight-week-old overnight-fasted ICR mice were administered powdered or LFED soybean. Mice were euthanized at 7, 15, 30, 60, 90, and 120 min after soybean intake. We have demonstrated that LFED soybean administration was quicker in stimulating muscle protein synthesis by activating mammalian target of rapamycin (mTOR) signaling than orally ingesting untreated soybean in the gastrocnemius muscle. These results suggested that LFED soybean is a more efficient source of nutrition for muscle hypertrophy than untreated soybean. Schema for acute effects of lactic acid-fermented and enzyme-digested soybean on protein synthesis via mTOR signaling in the skeletal muscle