Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013

• Published in: Veterinary microbiology Vol. 177; no. 1-2; pp. 201 - 205
• Main Authors: Ducatez, Mariette F., Awoume, Félix, Webby, Richard J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.05.2015; Elsevier
• Subjects: Animal biology; Animals; Base Sequence; Cell Line; Dogs; H1N1; Hemagglutinins; Humans; Influenza A Virus, H1N1 Subtype - genetics; Influenza virus; Interspecies transmission; Life Sciences; Microbiology and Parasitology; Molecular Sequence Data; Orthomyxoviridae Infections - epidemiology; Orthomyxoviridae Infections - transmission; Orthomyxoviridae Infections - veterinary; Pandemic; Reverse Transcriptase Polymerase Chain Reaction; Santé publique et épidémiologie; Sequence Analysis, DNA; Swine; Swine Diseases - epidemiology; Swine Diseases - transmission; Swine Diseases - virology; Togo; Veterinary medicine and animal Health; Virology; Pandemic; Interspecies transmission; Togo; Influenza virus; Swine; H1N1
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2015.02.028

•First detection of swine influenza virus in Togo.•2009 H1N1 pandemic influenza virus in pigs in Togo.•Human-to-swine transmission of 2009 H1N1 pandemic influenza virus in Togo. We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lomé in Togo. Influenza A virus genome was detected by RT-PCR in 2.5–12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eight genes similar to A(H1N1)pdm09 virus genes circulating in humans in 2012–2013, suggesting human-to-swine transmission of the pathogen.