Evaluation of phosphorus starvation inducible genes relating to efficient phosphorus utilization in rice

• Published in: Plant and soil Vol. 269; no. 1/2; pp. 81 - 87
• Main Authors: Shinano, T., Nanamori, M., Dohi, M., Wasaki, J., Osaki, M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Dordrecht Springer 01.02.2005; Springer Nature B.V
• Subjects: 2nd International Symposium on Phosphorus Dynamics in the Soil-Plant Continuum; Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; Biological and medical sciences; Chloroplasts; Classical and quantitative genetics. Population genetics. Molecular genetics; Dehydrogenase; Enzymes; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetics and breeding of economic plants; Messenger RNA; Molecular genetics; Oryza sativa; Phosphates; Phosphorus; Phosphorus metabolism; Plant cells; Plants; Polymerase chain reaction; Rice; Varietal selection. Specialized plant breeding, plant breeding aims; Phosphates; Monocotyledones; PEP/phosphate transporter; Genomics; Biological transport; Environmental factor; Lyases; real-time PCR; Inorganic element; Cereal crop; mRNA expression; Glyceraldehyde-3-phosphate dehydrogenase (NADP; Oryza sativa; Messenger RNA; Gene; Gramineae; Carboxy-lyases; Angiospermae; Phosphoenolpyruvate carboxylase; Carrier protein; Adaptation; Translocation; Gene physiology; Macronutrient(mineral); Evaluation; Deprivation; Nutrition; Pyruvate kinase; phosphorus deficiency; Enzyme; Induction; Transferases; Deficiency; Nutrient recovery; Inorganic ion; Glyceraldehyde-3-phosphate dehydrogenase [NAD(P)] (phosphorylating); Gene expression; Real time; Polymerase chain reaction; Carbon-carbon lyases; Inorganic anion; phosphorylating; Genetic improvement; Phosphorus compound; Spermatophyta; Oxidoreductases; Molecular biology; Soil plant relation
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-004-5026-2

Plants develop strategies to recycle phosphorus so that all organs receive adequate amounts of phosphorus, especially new growing organs. To evaluate the metabolic adaptation of rice plants under phosphorus deficient conditions, we selected several genes related to phosphorus utilization efficiency in the cell. Phosphoenolpyruvate carboxylase, triose phosphate translocator, phosphoenolpyruvate/phosphate translocator (PPT), pyruvate kinase, NAD dependent glyceraldehyde-3-phosphate dehydrogenase, and NADP dependent glyceraldehyde-3-phosphate dehydrogenase were selected because of their important roles in phosphorus utilization by the cell, and because they are part of the proposed bypass pathways by which the cells save phosphate. The most dramatic change was observed in the expression level of PPT (which transports phosphoenolpyruvate (PEP) from the cytosol into the chloroplast); thus we believe that PEP may play an important role in maintaining carbon metabolism under phosphate deficient conditions.