Meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae
Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tet...
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Published in | Genetics (Austin) Vol. 186; no. 2; pp. 537 - 550 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Genetics Society of America
01.10.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tetrads with four viable spores, two of the spores had two copies of a given homolog and two spores had only one copy. Chromosomes segregated randomly into viable spores without preferences for generating near haploid or near diploid spores. Using single-nucleotide polymorphisms, we showed that, in most tetrads, all three pairs of homologs recombined. Strains derived from some of the aneuploid spore colonies had very high frequencies of mitotic chromosome loss, resulting in genetically diverse populations of cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Communicating editor: R. S. Hawley Supporting information is available online at http://www.genetics.org/cgi/content/full/genetics.110.121533/DC1. |
ISSN: | 1943-2631 0016-6731 1943-2631 |
DOI: | 10.1534/genetics.110.121533 |