Peel LTP (Pru p 3) – the major allergen of peach – is methylated. A proteomic study

• Published in: Food chemistry Vol. 141; no. 3; pp. 2765 - 2771
• Main Authors: Larocca, Marilena, Martelli, Giuseppe, Grossi, Gerarda, Padula, Maria Carmela, Riccio, Paolo, Rossano, Rocco
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Allergens; Allergens - chemistry; Allergens - metabolism; Antigens, Plant - chemistry; Antigens, Plant - metabolism; Biological and medical sciences; Food toxicology; Fruit - chemistry; Fruit - metabolism; LTP; MALDI-TOF; Mass Spectrometry; Medical sciences; Methylation; Plant Proteins - chemistry; Plant Proteins - metabolism; Protein purification; Proteomics; Prunus; Prunus - chemistry; Prunus - metabolism; Prunus persica; Reversed-phase chromatography; Toxicology; Allergens; LTP; MALDI-TOF; Methylation; Protein purification; Reversed-phase chromatography; Reversed phase chromatography; Purification; Proteomics; Protein; Allergen
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.04.082

•We report on a new procedure for one-step purification of LTP.•Presence of the same protein with identical amino acid sequence in all genotypes.•Presence of a double methylation on the arginine residues in LTP purified from peel. Lipid transfer protein (LTP, Pru p 3) is the major allergen of peach (Prunus persica), and is in a greater abundance in the peel than in the pulp of the fruit. Peel LTP is more allergenic than pulp LTP, but it is not clear whether this is due to its specific allergenic properties or to its higher concentration. In this study, we have used a new one-step, rapid procedure for the purification of LTP from peel and pulp of four peach varieties [Gladys (white flesh), California (nectarine yellow flesh), Plusplus (yellow flesh), Red Fair (nectarine yellow flesh)] harvested in a field grown in Southern Italy. Purification was based on miniature reversed-phase chromatography, a procedure suitable for proteomic study. Proteomic analysis of purified LTPs revealed that the amino acid sequence of LTP was identical in all peach genotypes but, for the first time, peel LTP was found to be methylated.