Identification of the pore forming element of Semliki Forest virus spikes

Pore formation at mildly acidic pH by SFV spike proteins was investigated using isolated and modified virions. Modification of the virions was performed by limited proteolysis in presence of octylglucoside and resulted in the formation of E1 particles and spikeless particles, respectively. Pore form...

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Bibliographic Details
Published inFEBS letters Vol. 375; no. 1; pp. 134 - 136
Main Authors Spyr, Christian A., Käsermann, Fabian, Kempf, Christoph
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 13.11.1995
Subjects
Aedes
Animals
Chlorides - pharmacology
Clone Cells
E1 protein
Electrophoresis, Polyacrylamide Gel
Glucosides
Hydrogen-Ion Concentration
Kinetics
Pore formation
Propidium - metabolism
Semliki Forest virus
Semliki forest virus - physiology
Viral Envelope Proteins - analysis
Viral Envelope Proteins - antagonists & inhibitors
Viral Envelope Proteins - metabolism
Zinc Compounds - pharmacology
E1 protein
Semliki Forest virus
Pore formation
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Summary:Pore formation at mildly acidic pH by SFV spike proteins was investigated using isolated and modified virions. Modification of the virions was performed by limited proteolysis in presence of octylglucoside and resulted in the formation of E1 particles and spikeless particles, respectively. Pore formation was detected by measuring the influx of propidium iodide into the viral particles. The results obtained clearly showed that the presence of E1 alone is sufficient to promote pore formation at mildly acidic pH. Thus E1 represents the pore forming element of the viral spike proteins.
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01197-M