The expression of esterase S gene of Drosophila virilis in Drosophila melanogaster

• Published in: FEBS letters Vol. 360; no. 2; pp. 194 - 196
• Main Authors: Sergeev, Pavel V., Panin, Vladislav M., Pavlova, Galina V., Kopantseva, Marina R., Shostak, Natalia G., Bashkirov, Victor N., Georgiev, Georgii P., Korochkin, Leonid I.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 27.02.1995
• Subjects: Amino Acid Sequence; Animals; Animals, Genetically Modified; Base Sequence; BSA, bovine serum albumin; Drosophila - enzymology; Drosophila - genetics; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophila virilis; est6, esterase 6; Esterase; Esterases - genetics; estS, esterase S; Female; Gene Expression Regulation, Enzymologic; Genes, Insect; Male; Molecular Sequence Data; Regulation of expression; Regulatory Sequences, Nucleic Acid; RNA, Messenger - genetics; SDS, sodium dodecyl sulfate; Sequence Alignment; Sequence Homology, Amino Acid; Tissue Distribution; Transfection; BSA, bovine serum albumin; est6, esterase 6; Esterase; estS, esterase S; Regulation of expression; Drosophila virilis; SDS, sodium dodecyl sulfate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)00102-F

Drosophila melanogaster was transformed with the esterase S gene from Drosophila virilis. This gene is strongly activated in ejaculatory bulbs of mature males of Drosophila virilis. The closely related gene from Drosophila melanogaster is activated in ejaculatory ducts. The tissue- and stage-specific expression of incomplete genomic copy of the esterase S gene integrated into the Drosophila melanogaster genome is the same as in Drosophila virilis. These data show that tissue and stage specificity is determined by relatively small 5′ regulatory region of the esterase S gene. The comparison between deduced amino-acid sequences of the esterase S of Drosophila virilis and esterase 6 of Drosophila melanogaster was performed. These sequences revealed 50% homology.