N‐Cadherin Is Expressed by Putative Stem/Progenitor Cells and Melanocytes in the Human Limbal Epithelial Stem Cell Niche

• Published in: Stem cells (Dayton, Ohio) Vol. 25; no. 2; pp. 289 - 296
• Main Authors: Hayashi, Ryuhei, Yamato, Masayuki, Sugiyama, Hiroaki, Sumide, Taizo, Yang, Joseph, Okano, Teruo, Tano, Yasuo, Nishida, Kohji
• Format: Journal Article
• Language: English
• Published: Bristol John Wiley & Sons, Ltd 01.02.2007
• Subjects: Adult; Animals; Biomarkers - metabolism; Cadherins - metabolism; Cell Differentiation - genetics; Cell Proliferation; Colony-Forming Units Assay; Corneal epithelium; Epithelium, Corneal - cytology; Epithelium, Corneal - metabolism; Flow Cytometry; Gene Expression Regulation; Humans; Limbal epithelium; Limbus Corneae - cytology; Limbus Corneae - metabolism; Melanocyte; Melanocytes - cytology; Melanocytes - metabolism; Mice; Middle Aged; Models, Biological; NIH 3T3 Cells; N‐cadherin; Protein Transport; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem cell; Stem Cells - cytology; Stem Cells - metabolism
• ISSN: 1066-5099; 1549-4918
• DOI: 10.1634/stemcells.2006-0167

Corneal epithelial stem cells are known to be localized to the basal layer of the limbal epithelium, providing a model system for epithelial stem cell biology; however, the mechanisms regarding the maintenance of these stem cells in their specialized niche remain poorly understood. N‐cadherin is a member of the classic cadherin family and has previously been demonstrated to be expressed by hematopoietic stem cells. In the present study, we demonstrate that N‐cadherin is expressed by putative stem/progenitor cells, as well as melanocytes, in the human limbal epithelial stem cell niche. In addition, we demonstrate that upon in vitro culture using 3T3 feeder layers, loss of N‐cadherin expression occurs with cell proliferation. These results indicate that N‐cadherin may be a critical cell‐to‐cell adhesion molecule between corneal epithelial stem/progenitor cells and their corresponding niche cells in the limbal epithelium.