Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

• Published in: FEBS letters Vol. 198; no. 2; pp. 315 - 320
• Main Authors: Mazurek, N., Weskamp, G., Erne, P., Otten, U.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 31.03.1986
• Subjects: 12-O-Tetradecanoylphorbol 13-acetate; Animals; Ca 2; Ca2; Calcium - analysis; Degranulation; Dose-Response Relationship, Drug; Histamine Release - drug effects; In Vitro Techniques; Lysophospholipids; Male; Mast Cells - analysis; Mast Cells - drug effects; Nerve growth factor; Nerve Growth Factors - pharmacology; Phosphatidylserines - pharmacology; Rat peritoneal mast cell, Rat leukemia cell line; Rats; Rats, Inbred Strains; Tetradecanoylphorbol Acetate - pharmacology; 12-O-Tetradecanoylphorbol 13-acetate; Degranulation; Nerve growth factor; Rat peritoneal mast cell, Rat leukemia cell line; Ca 2
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80428-8

Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca 2+]). It is suggested that NGF has a unique mode of action independent of ([Ca 2+]) i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca 2+] i rise.