DNA binds neutrophil elastase and mucus proteinase inhibitor and impairs their functional activity
DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on el...
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Published in | FEBS letters Vol. 361; no. 2; pp. 265 - 268 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier B.V
20.03.1995
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Subjects | |
Online Access | Get full text |
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Summary: | DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on elastin. DNA strongly increases
k
diss and
K
i for the inhibition of elastase by mucus proteinase inhibitor
▪ The above effects are all salt-dependent. At physiological ionic strength, DNA is a potent inhibitor of the elastolytic activity of elastase and increases
k
diss and
K
i for the elastase-mucus proteinase inhibitor interaction 160-fold and 100-fold, respectively. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/0014-5793(95)00173-7 |