Altered Distribution of Mitochondria and Actin Fibers in 3T3 Cells Cultured on Microcarriers

• Published in: Biotechnology progress Vol. 8; no. 6; pp. 572 - 575
• Main Authors: Laney, Tracey V.Du, Cherry, Robert S., Coppinger, Justine A., Truskey, George A.
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01.11.1992; American Institute of Chemical Engineers
• Subjects: 3T3 Cells - ultrastructure; Actins - ultrastructure; Animal cells; Animals; Biological and medical sciences; Biotechnology; Cell Division; Cytological Techniques; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Kinetics; Methods. Procedures. Technologies; Mice; Miscellaneous; Mitochondria - ultrastructure; Staining and Labeling; Cell culture; Agitation; Rodentia; Contractile protein; Actins; 3T3-Cell line; Vertebrata; Mitochondria; Mammalia; Microcarrier cell culture; Mouse; Fluorescent labelling; Animal; Distribution; Cytoskeleton; Fibroblast
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp00018a600

The mitochondria and actin fibers of 3T3 fibroblasts cultured on microcarriers in spinner flasks were visualized using fluorescent stains. In contrast to cells grown on planar surfaces under static or steady laminar flow conditions, cells exposed to higher levels of turbulent agitation do not form actin stress fibers. Greater agitation also leads to a more diffuse appearance of the mitochondria and a wider distribution of them throughout the cytoplasm. This response may indicate damaged mitochondria, as similar results have been reported for chemical toxins.