Calcium transported to isolated rat liver nuclei by nicotinamide adenine dinucleotide is insensitive to thapsigargin

Calcium uptake by isolated nuclei was mediated by nicotinamide adenine dinucleotide. Oxidized nicotinamide nucleotide analogues were more effective mediators of nuclear calcium uptake. Thapsigargin inhibited ATP-mediated nuclear calcium transport without affecting NAD-mediated nuclear calcium uptake...

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Bibliographic Details
Published inFEBS letters Vol. 387; no. 1; pp. 85 - 88
Main Authors Matter, N., Malviya, A.N.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 27.05.1996
Subjects
Animals
Biological Transport
Ca 2+-ATPase
Ca2+-ATPase
Calcium - metabolism
Calcium Radioisotopes
Cell Nucleus - metabolism
Liver - cytology
Liver - drug effects
Liver - metabolism
NAD
NAD - metabolism
Nuclear calcium
Rats
Terpenes - pharmacology
Thapsigargin
NAD
Thapsigargin
Nuclear calcium
Ca 2+-ATPase
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Summary:Calcium uptake by isolated nuclei was mediated by nicotinamide adenine dinucleotide. Oxidized nicotinamide nucleotide analogues were more effective mediators of nuclear calcium uptake. Thapsigargin inhibited ATP-mediated nuclear calcium transport without affecting NAD-mediated nuclear calcium uptake. Whilst DBHQ did not influence ATP-induced calcium transport, it did stimulate NAD-mediated nuclear calcium entry. Calcium channel blockers did not influence the action of NAD. This study provides a further mechanism for nuclear calcium transport regulated by changes in the cytosolic NAD +/NADH ratio.
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00464-4