Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin

The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M f strongly decreases at the expense of M s. Kineti...

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Bibliographic Details
Published inFEBS letters Vol. 386; no. 1; pp. 55 - 59
Main Authors Luchian, Tudor, Tokaji, Zsolt, Dancsházy, Zsolt
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 13.05.1996
Subjects
Absorption
bacteriorhodopsin
Bacteriorhodopsins - physiology
Bacteriorhodopsins - radiation effects
Halobacterium halobium
intermediates of the photocycle
Kinetics
Light
Light intensity dependence
M intermediate decay
Mf, Ms, N and O
Models, Biological
Oxygen - metabolism
Parallel pathways
Photochemistry
Photocooperativity
Photocycle model
Time Factors
M intermediate decay
BR
M f, M s, N and O
Light intensity dependence
Photocycle model
Parallel pathways
Halobacterium halobium
Photocooperativity
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Summary:The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M f strongly decreases at the expense of M s. Kinetic calculations and the optical anisotropy of O show that O can be formed only from M f although M f and O have different light intensity dependences. In order to resolve the apparent contradiction, a phenomenologically new cooperative regulatory mechanism seems to be necessary.
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00391-2