Effects of N-acetylcysteine on TEGDMA- and HEMA-induced suppression of osteogenic differentiation of human osteosarcoma MG63 cells

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 98B; no. 2; pp. 300 - 307
• Main Authors: Kim, Na Ryoung, Lim, Bum-Soon, Park, Hee Chul, Son, Kyung Mi, Yang, Hyeong-Cheol
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2011; Wiley-Blackwell
• Subjects: Acetylcysteine - pharmacology; Active Transport, Cell Nucleus; Biocompatibility; Biological and medical sciences; Biomedical materials; cell differentiation; Cell Differentiation - drug effects; Cell Line, Tumor; cell-material interaction; Composite Resins - pharmacology; Dental materials; dental resin monomer; Differentiation; Diseases of the osteoarticular system; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Humans; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Methacrylates - pharmacology; NF-E2-Related Factor 2 - metabolism; Osteogenesis; Osteosarcoma - pathology; Oxidative Stress; Polyethylene Glycols - pharmacology; Polymers; Polymethacrylic Acids - pharmacology; Resins; Stresses; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Triethylene glycol dimethacrylate; Tumors of striated muscle and skeleton; Human; Oxidative stress; Sarcoma; Diseases of the osteoarticular system; Acetylcysteine; Hydroxyethyl methacrylate copolymer; Malignant tumor; Cell differentiation; Osteoarticular system; Crosslinked copolymer; Dental restoration; cell―material interaction; Osteosarcoma; Biomaterial; Methacrylate copolymer; Bone; dental resin monomer; Osteogenesis; Cancer; Biomedical engineering
• ISSN: 1552-4973; 1552-4981; 1552-4981
• DOI: 10.1002/jbm.b.31852

Triethyleneglycol dimethacrylate (TEGDMA) and 2‐hydroxyethyl methacrylate (HEMA) are major resinous components of dental restorative materials and dentin bonding adhesives. Resin monomers are known to cause cytotoxicity in mammalian cells via oxidative stress and inhibit differentiation of dental pulp cells and osteoblasts. This study was aimed to investigate whether oxidative stress was involved in the inhibition of TEGDMA‐ and HEMA‐induced differentiation. TEGDMA and HEMA reduced alkaline phosphatase (ALP) activity and the mRNA expression of the osteopontin (OPN) gene in MG63 cells at noncytotoxic concentrations. On the other hand, N‐acetylcysteine (NAC) did not affect ALP activity at concentrations below 10 mM. Reduced ALP activity and OPN mRNA expression by TEGDMA were partially recovered via cotreatment with NAC. However, NAC did not exhibit significant effects in HEMA‐treated cells. Glutathione (GSH) levels were also down‐regulated by both TEGDMA and HEMA. The addition of NAC induced the partial recovery of GSH in cells treated with 0.5 mM TEGDMA. On the other hand, the levels of GSH in HEMA‐treated cells were not affected by NAC. These results suggest that oxidative stress is involved in the suppression of differentiation by TEGDMA. Translocation of Nrf2 from the cytoplasm to the nucleus has been known to play a role in the suppression of osteogenic differentiation by oxidative stress. However, Nrf2 did not move into the nucleus in resin monomer‐treated MG63 cells, suggesting the contribution of other signaling pathways to the suppressive effects of resin monomers. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.