Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering

• Published in: Journal of biomedical materials research. Part A Vol. 98A; no. 3; pp. 332 - 341
• Main Authors: Yuan, Tun, Li, Kuifeng, Guo, Likun, Fan, Hongsong, Zhang, Xingdong
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2011; Wiley-Blackwell
• Subjects: allogeneic engineered cartilage; Animals; Biological and medical sciences; Biotechnology; Cartilage - cytology; Cattle; Cells, Cultured; collagen scaffolds; Collagen Type I - immunology; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; immune rejection; immunological properties; Industrial applications and implications. Economical aspects; Interferon-gamma - immunology; Lymphocyte Culture Test, Mixed; Major Histocompatibility Complex; Medical sciences; Mesenchymal Stem Cell Transplantation; mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - immunology; Miscellaneous; Rabbits; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Tissue Engineering; Tissue Scaffolds - chemistry; Transplantation, Homologous; Graft(material); Tissue engineering; Stem cell; Glycoprotein; allogeneic engineered cartilage; Mesenchymal cell; Scaffold; Rejection; immunological properties; Articular cartilage; Collagen; mesenchymal stem cells; Animal protein; Biomaterial; immune rejection; collagen scaffolds; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.33121

Influence of the structures of some collagen scaffolds on immunological properties of the seeded allogeneic mesenchymal stem cells (MSCs) was studied in this article. Hydrogels, sponge, and membrane were prepared from type‐I collagen. These scaffolds were seeded with neonatal rabbit MSCs and cultured for different periods. Changes of the immunological properties associated with different scaffolds were analyzed and compared. It was found that the expression of major histocompatibility complex (MHC) class I and II molecules on MSCs increased gradually in all scaffolds, but the least increment was recorded in hydrogels. Mixed lymphocyte reactions (MLR) showed that the MSC‐hydrogel constructs invoked considerably low allogeneic lymphocytes proliferation. Even in presence of interferon‐γ (IFN‐γ), the hydrogels with higher concentration gave comparatively lower increment of MHC‐II expression and allogeneic lymphocytes proliferation. These results suggest that different scaffold structures may provide different microenvironments and extents of isolation from the host immune system for the seed cells, thereby affecting their immunological properties. Therefore, scaffold structures may modulate the immunological properties of tissue‐engineered cartilage with allogeneic cells. Hydrogels, especially which were prepared from higher collagen concentrations, were found to be a promising scaffold structure, from the perspective of avoiding severe immune rejection. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.