Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

• Published in: FEBS letters Vol. 379; no. 1; pp. 107 - 111
• Main Authors: Tanabe, Osamu, Nagase, Terumasa, Murakami, Takehiko, Nozaki, Hideto, Usui, Hirofumi, Nishito, Yasumasa, Hayashi, Hideyuki, Kagamiyama, Hiroyuki, Takeda, Masao
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 22.01.1996
• Subjects: 74-kDa regulatory subunit; Amino Acid Sequence; Animals; base pair; Base Sequence; Binding Sites; Cerebral Cortex - enzymology; Cloning; Cloning, Molecular; DNA, Complementary - genetics; Escherichia coli - genetics; Human cerebral cortex; Human erythrocyte; Humans; IPTG; isopropylthio-β-d-galactoside; kilobase; Male; Molecular Sequence Data; Molecular Weight; PAGE; PCR; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - genetics; polyacrylamide gel electrophoresis; polymerase chain reaction; polyvinylidene difluoride; PP2A; Protein Conformation; Protein Phosphatase 2; Protein phosphatase 2A; PVDF; Rats; Rats, Wistar; Repetitive Sequences, Nucleic Acid; RNA, Messenger - genetics; RNA, Messenger - metabolism; SH3; Src homology 3; Tissue Distribution; Human erythrocyte; 74-kDa regulatory subunit; Cloning; PP2A; PAGE; PVDF; bp; kb; IPTG; Human cerebral cortex; PCR; Protein phosphatase 2A; SH3
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)01500-0

Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an M r 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRNA of about 2.9 kb was ubiquitously expressed in rat tissues.