Effectiveness of erythrosine-mediated photodynamic antimicrobial chemotherapy on dental plaque aerobic microorganisms: A randomized controlled trial

• Published in: Journal of Indian Society of Periodontology Vol. 21; no. 3; pp. 210 - 215
• Main Authors: Bhat, Manohar, Acharya, Swathi, Prasad, Kakarla, Kulkarni, Raghavendra, Bhat, Anithraj, Bhat, Devikripa
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.05.2017; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Aerobic bacteria; Aerobic microorganisms; Analysis; Antibiotics; Antimicrobial agents; Biofilms; Chemotherapy; Clinical trials; Dental plaque; Dental restorative materials; Dentistry; Diagnosis; Disease; Hospitals; Jaw; Lasers; Light; Microorganisms; Oral diseases; Original; Photochemotherapy; Photodynamic therapy; Sample size; Streptococcus mutans; Teeth; Dental plaque; erythrosine; photodynamic antimicrobial chemotherapy
• ISSN: 0972-124X; 0975-1580
• DOI: 10.4103/jisp.jisp_157_17

Background: Dental plaque is one of the predominant causes of major oral diseases. Although mechanical and chemical methods are extensively followed to control the development of plaque, plaque-related diseases still persist. Therefore, this necessitates for alternative measures of plaque control, one such alternative is photodynamic antimicrobial chemotherapy (PACT). Materials and Methods: Split mouth randomized clinical trial (CTRI/2017/03/008239) was conducted on 30 participants who reported to the hospital. Participants were asked to rinse their mouth for 1 min using 10 ml of 25 μM erythrosine solutions. Same tooth on both quadrants of the same jaw are selected as the test and control. Intervention used was halogen-based composite curing light with wavelength of 500-590 nm. Plaque sample from the control tooth and test tooth was collected before and after exposure, respectively, and sent to microbiological laboratory for colony count. Results: Logarithmic mean and standard deviation of control group with 102 dilutions of aerobic microbial count were found to be 5.34 ± 0.94, and for experimental group, it was 4.47 ± 1.37. The statistical difference between mean CFU values between aerobic bacterial counts was significant (P = 0.006). Conclusions: Erythrosine-mediated PACT reduces the extent of dental plaque microbial count and has a potential preventive and therapeutic use in day-to-day life and dental clinics.