RADIOIMMUNOASSAY FOR THE DETERMINATION OF GINSENG SAPONIN, GINSENOSIDE RG1

Radioimmunoassay method for the determination of ginsenoside Rg1, one of the major saponins of Panax ginseng root, was established. The side chain of ginsenoside Rg1 was modified and coupled to BSA. 125I-Labelled compound of ginsenoside Rg1 was prepared from a ginsenoside Rg1-tyramine conjugate. Imm...

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Published inChemical & pharmaceutical bulletin Vol. 30; no. 5; pp. 1907 - 1910
Main Authors Sankawa, Ushio, Sung, Chung Ki, Han, Byung Hoon, Akiyama, Toshiyuki, Kawashima, Koichiro
Format Journal Article
LanguageEnglish
Published The Pharmaceutical Society of Japan 01.01.1982
Subjects
dammarane
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Summary:Radioimmunoassay method for the determination of ginsenoside Rg1, one of the major saponins of Panax ginseng root, was established. The side chain of ginsenoside Rg1 was modified and coupled to BSA. 125I-Labelled compound of ginsenoside Rg1 was prepared from a ginsenoside Rg1-tyramine conjugate. Immunization of rabbits with the hapten-BSA conjugate gave antiserum possessing high affinity and specificity toward ginsenoside Rg1. Ginsenoside Rg1 can be determined by the established radioimmunoassay method over a range of 250 pg-10 ng per tube.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.30.1907