Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis

Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators-auxin...

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Published inFrontiers in plant science Vol. 13; p. 830840
Main Authors Wang, Ming, Ogé, Laurent, Pérez Garcia, Maria-Dolores, Launay-Avon, Alexandra, Clément, Gilles, Le Gourrierec, Jose, Hamama, Latifa, Sakr, Soulaiman
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers 17.03.2022
Frontiers Media S.A
Subjects
auxin
Biochemistry, Molecular Biology
Botanics
bud outgrowth
Genomics
Life Sciences
Plant Science
signaling pathway
sucrose
TOR-kinase
Vegetal Biology
TOR-kinase
signaling pathway
auxin
sucrose
bud outgrowth
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Summary:Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators-auxin and sugar-antagonistically regulate plant branching. However, little is known regarding the molecular mechanisms involved in this crosstalk. We carried out two complementary untargeted approaches-RNA-seq and metabolomics-on explant stem buds fed with different concentrations of auxin and sucrose resulting in dormant and non-dormant buds. Buds responded to the combined effect of auxin and sugar by massive reprogramming of the transcriptome and metabolome. The antagonistic effect of sucrose and auxin targeted several important physiological processes, including sink strength, the amino acid metabolism, the sulfate metabolism, ribosome biogenesis, the nucleic acid metabolism, and phytohormone signaling. Further experiments revealed a role of the TOR-kinase signaling pathway in bud outgrowth through at least downregulation of ( ). These new findings represent a cornerstone to further investigate the diverse molecular mechanisms that drive the integration of endogenous factors during shoot branching.
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Edited by: Serena Varotto, University of Padua, Italy
This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science
Reviewed by: Tesfamichael Kebrom, Prairie View A&M University, United States; Jacob Oliver Brunkard, University of California, Berkeley, United States
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.830840