Autoantibodies Directed Against CD43 Molecules With an Altered Glycosylation Status on Human Immunodeficiency Virus Type 1 (HIV-1)-Infected CEM Cells Are Found in All HIV-1+ Individuals

• Published in: Blood Vol. 86; no. 6; pp. 2302 - 2311
• Main Authors: Giordanengo, Valerie, Limouse, Martine, Roure, Laurence Desroys Du, Cottalorda, Jacqueline, Doglio, Alain, Passeron, Alain, Fuzibet, Jean-Gabriel, Lefebvre, Jean-Claude
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 15.09.1995; The Americain Society of Hematology
• Subjects: Acquired Immunodeficiency Syndrome - immunology; Antigens, CD; Arthritis, Rheumatoid - immunology; Autoantibodies - immunology; Autoantigens - chemistry; Autoantigens - immunology; Biological and medical sciences; Cell Line; Cross Reactions; Glycosylation; Hepatitis B - immunology; HIV Infections - immunology; HIV-1; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunoglobulin G - immunology; Immunoglobulin M - immunology; Immunopathology; Leukosialin; Lupus Erythematosus, Systemic - immunology; Medical sciences; Molecular Weight; N-Acetylneuraminic Acid; Sialic Acids - analysis; Sialoglycoproteins - chemistry; Sialoglycoproteins - immunology; T-Lymphocytes - immunology; T-Lymphocytes - virology; Human; Immunopathology; HIV-1 virus; Antibody; Retroviridae; Autoantibody; Antigen antibody reaction; Exploration; AIDS; Glycosylation; Immune deficiency; In vitro; Cell surface; Infection; Virus; Antigen; Autoantigen; Viral disease; Lentivirinae; Serum; Human immunodeficiency virus; Lymphocyte
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V86.6.2302.bloodjournal8662302

Autoantibodies to lymphocytes have been detected in sera from human immunodeficiency virus type 1 (HIV-1 (infected individuals, and several autoantigens have been described. Among them, hyposialylated CD43 has been shown to be a target for autoantibodies in up to 47% of HIV+ individuals. However, the corresponding autoantigen fie, the incompletely sialylated CD43) has not been isolated from blood cells of HIV-1-infected individuals. Recently, we have observed in vitro that HIV-1 productively or latently infected CEM cells (CEMLAI/NP) express CD43 molecules with modified glycosylation (mogly CD43). Using CEMLAI/NP cells, which do not express any structural viral antigen, we show now that all of the tested HIV+ sera from asymptomatic individuals, and up to 86% of those from subjects at the acquired immunodeficiency syndrome stage contain antibodies (mainly IgM and, to a lesser degree, IgG) that recognize the surface of CEMLAI/NP cells, and precipitate mogly CD43 molecules from the cells lysates. Taken together with our previous demonstration of altered glycosylation of CD43 from HIV-1-infected CEM cells in vitro, the constant anti-mogly CO43 autoimmune response observed from asymptomatic HIV-1+ subjects is likely to illustrate the occurence of an altered glycosylation in vivo of the major lymphocyte surface CD43 glycoprotein, associated with HIV-1 infection.