IS6110-based PCR methods for detection of Mycobacterium tuberculosis

Kent et al. detected Mycobacterium tuberculosis by a nested PCR test that amplified a 181-bp target located in insertion sequence IS6110, but they also encountered many false-positive results when the test was applied to isolates of mycobacteria that do not belong to the M. tuberculosis complex. Bec...

Full description

Saved in:
Bibliographic Details
Published inJournal of clinical microbiology Vol. 34; no. 5; pp. 1348 - 1349
Main Authors Mulcahy, G M, Kaminski, Z C, Albanese, E A, Sood, R, Pierce, M
Format Journal Article
LanguageEnglish
Published United States 01.05.1996
Subjects
DNA Transposable Elements
False Positive Reactions
Humans
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Polymerase Chain Reaction - methods
Tuberculosis - diagnosis
Tuberculosis - microbiology
Online AccessGet full text

Cover

More Information
Summary:Kent et al. detected Mycobacterium tuberculosis by a nested PCR test that amplified a 181-bp target located in insertion sequence IS6110, but they also encountered many false-positive results when the test was applied to isolates of mycobacteria that do not belong to the M. tuberculosis complex. Because the investigators attributed the false positives to homology between IS6110 and genomic DNA of the other mycobacterial species, they warned that false-positive PCR results could be a problem with some IS6110-based methods for detection of M. tuberculosis complex.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
SourceType-Other Sources-1
content type line 63
ObjectType-Correspondence-1
ObjectType-Commentary-2
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.34.5.1348-1349.1996