The Role of Caspase-3, Apoptosis-Inducing Factor, and B-cell Lymphoma-2 Expressions in Term Premature Rupture of Membrane

• Published in: Revista Brasileira de ginecologia e obstetrícia Vol. 40; no. 12; pp. 733 - 739
• Main Authors: Negara, Ketut Surya, Suwiyoga, Ketut, Pemayun, Tjokorda Gede Astawa, Sudewi, Anak Agung Raka, Astawa, Nyoman Mantik, Arijana, I Gusti Nyoman Kamasan, Tunas, Ketut
• Format: Journal Article
• Language: English
• Published: Brazil Thieme Revinter Publicações Ltda 01.12.2018; Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
• Subjects: Adult; apoptosis; apoptosis inducing factor; Apoptosis Inducing Factor - biosynthesis; B-cell lymphoma-2 expression; Case-Control Studies; Caspase 3 - biosynthesis; caspase-3; Female; Fetal Membranes, Premature Rupture - metabolism; Humans; OBSTETRICS & GYNECOLOGY; Original; Pregnancy; PROM; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; apoptosis; B-cell lymphoma-2 expression; PROM; apoptosis inducing factor; caspase-3
• ISSN: 0100-7203; 1806-9339; 1806-9339
• DOI: 10.1055/s-0038-1675611

Objective To determine the role of caspase-3, apoptosis-inducing factor (AIF), and B-cell lymphoma-2 (Bcl-2) expressions in term premature rupture of membrane (PROM). Methods An analytic observational study with case-control design was conducted, involving 52 subjects (37–42 weeks of gestation) who were divided into 2 groups: 26 cases of term delivery with PROM, and 26 controls of term delivery without PROM. The expressions of caspase-3, AIF, and Bcl-2 in the amniotic membrane were determined by immunohistochemistry. Data were analyzed using the chi-squared test. The risk of PROM was expressed by odds ratio (OR). Results There were no significant differences in age, parity and body mass index between the two groups (p > 0.05). High caspase-3 and AIF expressions increased the risk of PROM 17.64 times (OR = 17.64; 95% CI = 4.44–70.07; p = 0.001) and 9.45 times (OR = 9.45; 95% CI= 2.62–34.07; p = 0.001), respectively, while low Bcl-2 expression increased 10.39 times (OR = 10.39; 95% CI = 2.73–39.56; p = 0.001)the risk of PROM . Conclusion High caspase-3 and AIF expressions and low Bcl-2 expression were risk factors for term PROM. Caspase-dependent and independent pathways of apoptosis were involved in the mechanism of PROM in term pregnancy.