A novel system to monitor mitochondrial translation in yeast

The mitochondrial genome is responsible for the production of a handful of polypeptides that are core subunits of the membrane-bound oxidative phosphorylation system. Until now the mechanistic studies of mitochondrial protein synthesis inside cells have been conducted with inhibition of cytoplasmic...

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Bibliographic Details
Published inMicrobial cell Vol. 5; no. 3; pp. 158 - 164
Main Authors Suhm, Tamara, Habernig, Lukas, Rzepka, Magdalena, Kaimal, Jayasankar Mohanakrishnan, Andréasson, Claes, Büttner, Sabrina, Ott, Martin
Format Journal Article
LanguageEnglish
Published Austria Shared Science Publishers OG 13.01.2018
Subjects
Biochemistry
biokemi
flow cytometry
Microbiology
mitochondrial translation
Molecular Biology
strain engineering
superfolder GFP
mitochondrial translation
superfolder GFP
flow cytometry
strain engineering
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Summary:The mitochondrial genome is responsible for the production of a handful of polypeptides that are core subunits of the membrane-bound oxidative phosphorylation system. Until now the mechanistic studies of mitochondrial protein synthesis inside cells have been conducted with inhibition of cytoplasmic protein synthesis to reduce the background of nuclear gene expression with the undesired consequence of major disturbances of cellular signaling cascades. Here we have generated a system that allows direct monitoring of mitochondrial translation in unperturbed cells. A recoded gene for superfolder GFP was inserted into the yeast ( ) mitochondrial genome and enabled the detection of translation through fluorescence microscopy and flow cytometry in functional mitochondria. This novel tool allows the investigation of the function and regulation of mitochondrial translation during stress signaling, aging and mitochondrial biogenesis.
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Please cite this article as: Tamara Suhm, Lukas Habernig, Magdalena Rzepka, Jayasankar Mohanakrishnan Kaimal, Claes Andréasson, Sabrina Büttner and Martin Ott (2018). A novel system to monitor mitochondrial translation in yeast. Microbial Cell 5(3): 158-164. doi: 10.15698/mic2018.03.621
Conflict of interest: The authors declare no conflict of interest.
ISSN:2311-2638
2311-2638
DOI:10.15698/mic2018.03.621