Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain

• Published in: BMC musculoskeletal disorders Vol. 21; no. 1; pp. 500 - 8
• Main Authors: Xiong, Zekang, Ding, Jun, Zhou, Jinge, Yao, Sheng, Zheng, Jin, Guo, Xiaodong
• Format: Journal Article
• Language: English
• Published: England BioMed Central 28.07.2020; BMC
• Subjects: Antibodies; Apoptosis; Back pain; Cell growth; Cell proliferation; Degeneration; Degenerative disc disease; Endplate degeneration; Gene expression; HIF-1α; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit; Hypoxia-inducible factor 1a; Intervertebral Disc; Intervertebral Disc Degeneration - diagnostic imaging; Intervertebral Disc Degeneration - surgery; Intervertebral discs; Investigations; Low Back Pain; Magnetic resonance imaging; Metabolism; Modic changes; Musculoskeletal diseases; Notch signaling pathway; Notch1 protein; Nucleus Pulposus; Proteins; Receptors, Notch; Signal Transduction; United States > US; China; Notch signaling pathway; HIF-1α; Modic changes; Nucleus pulposus; Endplate degeneration
• ISSN: 1471-2474; 1471-2474
• DOI: 10.1186/s12891-020-03505-w

The HIF-1α/Notch signaling pathway regulates cell proliferation, apoptosis, and metabolism in the intervertebral discs (IVDs) and is implicated in disc degeneration. The nucleus pulposus (NP) is an important structure adjacent to the IVDs. However, the role of the HIF-1α/Notch signaling pathway in NP cells obtained from patients with different Modic changes (MCs) remains unclear. The purpose of the present study was to investigate the role of HIF-1α and components of the Notch pathway in the NP obtained from patients with various MCs. A total of 85 NP tissue samples were collected from patients undergoing diskectomy for the treatment of low back pain. The NP tissues were divided into four groups based on the adjacent endplate degeneration, namely, MC I, II, III, and negative MC groups. The expression of HIF-1α and Notch-related components was measured and compared. The expression of HIF-1α, Notch1, and Notch2 was gradually increased in the MC I and MC II groups compared with that in the negative MC group. HIF-1α and Notch-related components were rarely detected in the MC III group. The expression of HIF-1α/Notch increased in the NP cells of patients with MC I and MC II. HIF-1α and Notch-related components are potential biomarkers and the HIF-1α/Notch signaling pathway may serve as a promising therapeutic target for disc degeneration in patients with MCs.