High Throughput T Epitope Mapping and Vaccine Development

Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libr...

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Bibliographic Details
Published inBioMed research international Vol. 2010; no. 2010; pp. 1 - 12
Main Authors Li Pira, Giuseppina, Ivaldi, Federico, Moretti, Paolo, Manca, Fabrizio
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Puplishing Corporation 01.01.2010
Hindawi Publishing Corporation
John Wiley & Sons, Inc
Subjects
Algorithms
Animals
Antigens
Automation
Epitope Mapping - methods
Epitopes, T-Lymphocyte
High-Throughput Screening Assays - methods
Humans
Immunology
Lymphocytes
Mice
Models, Immunological
Pathogens
Peptides
Proteins
Review
Vaccines
Workloads
Italy
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Summary:Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libraries to monitor specific cellular immunity in protected individuals, patients and vaccinees. Nevertheless, epitope mapping is a challenging task. In fact, large panels of overlapping peptides need to be tested with lymphocytes to identify the sequences that induce a T-cell response. Since numerous peptide panels from antigenic proteins are to be screened, lymphocytes available from human subjects are a limiting factor. To overcome this limitation, high throughput (HTP) approaches based on miniaturization and automation of T-cell assays are needed. Here we consider the most recent applications of the HTP approach to T epitope mapping. The alternative or complementary use of in silico prediction and experimental epitope definition is discussed in the context of the recent literature. The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost.
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Academic Editor: Yongqun Oliver He
ISSN:1110-7243
2314-6133
1110-7251
1110-7251
2314-6141
DOI:10.1155/2010/325720