Rabbit liver growth hormone receptor and serum binding protein. Purification, characterization, and sequence

• Published in: The Journal of biological chemistry Vol. 263; no. 16; pp. 7862 - 7867
• Main Authors: Spencer, S A, Hammonds, R G, Henzel, W J, Rodriguez, H, Waters, M J, Wood, W I
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.06.1988; American Society for Biochemistry and Molecular Biology
• Subjects: amino acid sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carrier Proteins - genetics; Carrier Proteins - isolation & purification; DNA - analysis; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; growth hormone; growth hormone-binding protein; Kinetics; liver; Liver - metabolism; Molecular Weight; Proteins; Rabbits; Receptors, Somatotropin - genetics; Receptors, Somatotropin - isolation & purification
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)68577-1

A putative growth hormone receptor from detergent-solubilized rabbit liver membranes and the growth hormone binding protein from rabbit serum have been purified 59,000- and 400,000-fold, respectively, primarily by affinity chromatography. Both purified proteins exhibit high affinity binding for human growth hormone; K alpha = 9-30 x 10(9) M-1 for the liver receptor and K alpha = 6 x 10(9) M-1 for the binding protein. The apparent molecular weight of the liver receptor is 130,000 by reduced sodium dodecyl sulfate gel electrophoresis, while that of the binding protein is 51,000. Both contain N-linked carbohydrate. The amino-terminal sequences of the liver growth hormone receptor and the serum binding protein were found to be the same, indicating that the binding protein corresponds to the extracellular domain of the liver receptor. Ubiquitin was found covalently linked to the liver receptor but not to the serum binding protein. The amino acid sequences of several peptides from the liver receptor were also determined after tryptic and V8 protease digestion.