Aptamer-based optical manipulation of protein subcellular localization in cells

• Published in: Nature communications Vol. 11; no. 1; p. 1347
• Main Authors: Xie, Sitao, Du, Yulin, Zhang, Yu, Wang, Zhimin, Zhang, Dailiang, He, Lei, Qiu, Liping, Jiang, Jianhui, Tan, Weihong
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 12.03.2020; Nature Publishing Group UK; Nature Portfolio
• Subjects: Aptamers; Aptamers, Nucleotide - genetics; Aptamers, Nucleotide - metabolism; Cell Nucleus - genetics; Cell Nucleus - metabolism; Cytoplasm - genetics; Cytoplasm - metabolism; Design modifications; Genetics; Humans; I.R. radiation; Information processing; Infrared radiation; Localization; Lysozyme; Modular design; NF-κB protein; Optics; Optogenetics; p53 Protein; Protein Transport; Proteins; RelA protein; Signal Transduction; Transcription Factor RelA - genetics; Transcription Factor RelA - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15113-2

Protein-dominant cellular processes cannot be fully decoded without precise manipulation of their activity and localization in living cells. Advances in optogenetics have allowed spatiotemporal control over cellular proteins with molecular specificity; however, these methods require recombinant expression of fusion proteins, possibly leading to conflicting results. Instead of modifying proteins of interest, in this work, we focus on design of a tunable recognition unit and develop an aptamer-based near-infrared (NIR) light-responsive nanoplatform for manipulating the subcellular localization of specific proteins in their native states. Our results demonstrate that this nanoplatform allows photocontrol over the cytoplasmic-nuclear shuttling behavior of the target RelA protein (a member of the NF-κβ family), enabling regulation of RelA-related signaling pathways. With a modular design, this aptamer-based nanoplatform can be readily extended for the manipulation of different proteins (e.g., lysozyme and p53), holding great potential to develop a variety of label-free protein photoregulation strategies for studying complex biological events.